Long-term boron-deficiency-responsive genes revealed by cDNA-AFLP differ between Citrus sinensis roots and leaves.

作者: Yi-Bin Lu , Yi-Ping Qi , Lin-Tong Yang , Jinwook Lee , Peng Guo

DOI: 10.3389/FPLS.2015.00585

关键词:

摘要: Seedlings of Citrus sinensis (L.) Osbeck were supplied with boron (B)-deficient (without H3BO3) or -sufficient (10 µM nutrient solution for 15 weeks. We identified 54 (38) and 38 (45) up (down)-regulated cDNA-AFLP bands (transcript-derived fragments, TDFs) from B-deficient leaves roots, respectively. These TDFs mainly involved in protein amino acid metabolism, carbohydrate energy nucleic cell transport, signal transduction, stress response defense. The majority the differentially expressed isolated only roots leaves, seven same GenBank ID both. In addition, ATP biosynthesis-related induced but unaffected leaves. Most associated transduction defense down-regulated up-regulated related to ubiquitination proteolysis except one TDF, while two detected roots. Thus, many differences existed long-term B-deficiency-responsive genes between conclusion, our findings provided a global picture differential responses occurring revealed new insight into different adaptive mechanisms C. B-deficiency at transcriptional level.

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