Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food.

摘要: A sensitive, specific procedure was developed for detecting Escherichia coli O157:H7 in food less than 20 h. The involves enrichment of 25 g 225 ml a selective medium 16 to 18 h at 37 degrees C with agitation (150 rpm). culture is applied sandwich enzyme-linked immunosorbent assay (ELISA) polyclonal antibody E. O157 antigen as the capture and monoclonal enterohemorrhagic serotypes O26:H11 detection antibody. ELISA can be completed within 3 sensitivity procedure, determined by using O157:H7-inoculated ground beef dairy products, including different varieties cheese, 0.2 0.9 cell per food. survey retail fresh farm raw milk samples this revealed that (2.8%) 107 11 (10%) 115 were positive O157:H7. Most-probable-number determinations populations 0.4 1.5 cells three samples. In addition being highly specific, rapid, easy perform amenable use laboratories performing routine microbiological testing.