Long non-coding RNA H19 mediates mechanical tension-induced osteogenesis of bone marrow mesenchymal stem cells via FAK by sponging miR-138.

摘要: Bone marrow mesenchymal stem cells (BMMSCs) provide the biological basis for bone reconstruction. Mechanical tension stimulation as a potent modulator is able to promote osteogenic capability of BMMSCs. Long non-coding RNAs (LncRNAs) competing endogenous (ceRNAs) microRNAs, are postulated regulate differentiation cells. However, mechanism how (whether) lncRNAs mediates tension-induced osteogenesis BMSCs still remains poor understood. Here, human BMMSCs (hBMMSCs) were subjected mechanical (10%, 0.5Hz). Results showed that could enhance and increase H19 expression. deficiency suppressed differentiation, demonstrating mediate in hBMMSCs. Besides, suppress miR-138 expression, down-regulated promoted Luciferase reporter assays illustrated had binding sites with miR-138, increased level, may act ceRNA also target PTK2,a gene encoding focal adhesion kinase (FAK). Up-regulated impaired FAK expression induced by tension. The relationship among H19, under condition was further studied. inhibited which be partly rescued knock-downing miR-138. In addition, defective knockdown. Taken together, this study indicated positive regulator hBMMSCs through acting then up-regulating downstream FAK.