DNA ploidy, proliferation, and neu-oncogene protein overexpression in breast carcinoma.

摘要: DNA content, proliferative activity (Ki-67 immuno-staining and S-phase fraction by flow cytometry), neu-oncogene overexpression were studied in 135 patients with invasive breast carcinoma. Image analysis cytometry of fresh tumors showed good correlation between the two methods yielded 39% diploid 61% aneuploid tumors. Aneuploidy, including tetraploidy, was significantly related to loss estrogen (p = 0.0002) progesterone 0.03) receptors, high histologic 0.014) nuclear less than 0.0001) grades, mitotic rate 0.0001). Immunohistochemical evaluation proliferation staining Ki-67 monoclonal antibody protein performed frozen tissue from 83 The score, quantitated CAS-200 image analyzer, correlated only moderately obtained linear regression (r 0.39, p 0.001). However, both these markers strongly Aneuploid tetraploid demonstrated higher scores fractions Neu-oncogene seen 24 (29%) overall much (38%) (50%) (7%). concentration positive region reported others not observed. also associated 0.016) 0.037).(ABSTRACT TRUNCATED AT 250 WORDS)