Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy

摘要: RNA interference or RNAi-based gene therapy for the treatment of HIV-1 infection has recently emerged as a highly effective antiviral approach. The lentiviral vector system is good candidate expression short hairpin RNAs (shRNA) in HIV-susceptible cells. However, this strategy can give rise to problems because anti-HIV shRNAs also target HIV-based system. In addition, there may be self-targeting shRNA-encoding sequences within genome producer cell. insertion microRNA (miRNA) cassettes introduce Drosha cleavage sites that will result destruction during production and/or transduction process. Here, we describe possible solutions these lentiviral-RNAi problems. We multiple shRNA establish combinatorial RNAi therapy.