Onchocerca - infected cattle produce strong antibody responses to excretory-secretory proteins released from adult male Onchocerca ochengi worms

摘要: The front line molecules from filarial worms and other nematodes or helminthes are their Excretory-Secretory (ES) products. Their interaction with the host cells, proteins immune system accounts for skin eye pathology hyposensitivity observed in human onchocerciasis. ES products adult worms’ crude extracts Onchocerca ochengi, a nematode that infects African zebu cattle, were utilized present study as model studying volvulus causes river blindness man. generated male female vitro analyzed poly acrylamide gel electrophoresis (PAGE) enzyme-linked immunosorbent assay (ELISA) using sera Onchocerca-infected cattle humans. collected herd had been exposed six years to natural transmission of spp. expressed reactivity was evaluated differences statistically Kruskal-Wallis rank Chi-square tests. electrophoretic analyses 156 O. ochengi two somatic three females 25 males revealed protein pattern showing pronounced bands at 15, 30–50 75 kDa 40–75 kDa extracts, respectively less than 100 kDa worms. Proteins recognized by IgG both Onchocerca-exposed Bovine serum antibodies reacted more strongly those Interestingly, reaction higher worms, suggesting which migrate one nodule another remain encapsulated intradermal nodules. This demonstrates and, particular, filariae may represent good source immunogenic potential vaccine candidates.