Synchronized Formation and Remodeling of Postsynaptic Densities: Long-Term Visualization of Hippocampal Neurons Expressing Postsynaptic Density Proteins Tagged with Green Fluorescent Protein
作者： Tatsuhiko Ebihara , Izumi Kawabata , Shinichi Usui , Kenji Sobue , Shigeo Okabe
DOI: 10.1523/JNEUROSCI.23-06-02170.2003
关键词:
摘要: To explore mechanisms governing the formation and remodeling of postsynaptic density (PSD), we used dissociated cultures hippocampal neurons isolated from transgenic embryos expressing green fluorescent protein (GFP)-tagged PSD proteins PSD-Zip45 (Homer 1c) PSD-95. Expression GFP-tagged molecules was stable, process PSDs could be followed for >1 week. A higher expression level GFP-PSD-Zip45 enabled us to quantitatively analyze amount clusters during development. Repetitive imaging same cell populations between 11 17 d in culture revealed an increase average cluster 0.32 0.73/μm. Newly generated dendrites rapidly acquired clusters, their reached parental within a few days. Temporal profiles showed variety patterns. Some monotonous whereas others complex patterns, including short decremental stages. Analysis long-term PSD-95-GFP confirmed that stages were not specific clusters. Comparison temporal among indicated synchronization both PSD-95 clustering individual cells. Furthermore, activation cAMP-dependent kinase suppressed remodeling. These observations suggest presence signaling can induce synchronized addition or elimination throughout dendritic arborization single neuron.
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