Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure.

摘要: Enzymatic synthesis methods for the fluorinated 5‘-triphosphate analogues 5F-UTP and 5F-CTP have been developed to facilitate 19F-labeling of RNAs biophysical studies. HIV-2 TAR were synthesized using these by in vitro transcription reactions T7 RNA polymerase. The uniform incorporation 5F-U or 5F-C into transcripts does not significantly alter structure thermodynamic stability. Fluorine observed homonuclear 19F−19F heteronuclear 19F−1H NOE experiments providing selective distance information are presented discussed. availability efficient 5F-UTP, first time, 5F-CTP, will use 5F-labeled structural, ligand binding, dynamic studies advantages 19F-labeling.