The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32.

摘要: Employing an expression cloning approach for tyrosine kinase substrates, we have previously isolated the coding sequence a novel putative EGFR substrate, eps15, from NIH3T3 fibroblasts. Eps15 displayed receptor-specific pattern of phosphorylation in vivo and was able to transform cells upon overexpression. To gain understanding eps15 function as well its role normal neoplastic proliferation, cloned human studied RNA protein, evolutionary conservation, chromosomal location. The close structural similarity with murine homologue is indicated by 89% 90% identity nucleotide predicted amino acid sequences, respectively. Using probe, demonstrate that member gene family highly conserved during evolution. An essential cell growth regulation underscored our observation ubiquitous at transcript protein level malignant cells. EPS15 locus maps chromosome 1p31-p32, region involved deletion neuroblastoma, translocations acute lymphoblastic leukemia, exhibiting fragile site.