Exosomes Are Unlikely Involved in Intercellular Nef Transfer

摘要: HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Several recent studies including ours have demonstrated that can be transferred to neighboring cells and alters the function of these cells. However, how intercellular transfer occurs in dispute. In current study, we attempted address this issue using several complementary strategies, a panel exosomal markers, human CD4+ T lymphocyte cell line Jurkat commonly used 293T. First, showed was from Nef-expressing or HIV-infected naive other non-Jurkat required membrane targeting density-dependent. Then, cell-cell contact-dependent, as exposure culture supernatants exosomes 293T led little detection target Jurkat. Thirdly, only detected associated with HIV virions but not acetylcholinesterase (AChE+) comparison, when it over-expressed 293T, detergent-insoluble AChE+/CD81low/TSG101low exosomes, detergent-soluble AChE-/CD81high/TSG101high exosomes. Lastly, microscopic imaging no significant vesicle-like structures out Taken together, results show are unlikely involved transfer. addition, study reveals existence two types exosomes: