Induction of ecto-5'-nucleotidase of rat cultured mesangial cells by interleukin-1 beta and tumour necrosis factor-alpha.

摘要: Because ecto-5'-nucleotidase activity of rat glomerular mesangial cells has been shown to increase upon interaction with macrophages in vitro, it was examined whether interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha), two macrophage-released cytokines, were responsible for this effect. IL-1 TNF-alpha stimulated cell 5'-nucleotidase a dose-dependent manner after treatment 24 hr. Maximum increases reached 4.5 times 1.7 basal values (20 U/ml) (25 ng/ml), respectively. The effects both cytokines additive. Stimulation by specific since the other ectoenzymes, such as Mg2(+)-ATPase, unchanged. Cycloheximide, blocker protein synthesis, suppressed cytokine-dependent activity. Cyclo-oxygenase inhibitors indomethacin ibuprofen inhibited approximately 50% cytokines. Their inhibitory effect abolished presence prostaglandin E2 (PGE2). In addition, PGE2 itself produced dose-related (0.1-10 microM) maximum 2.2 value. Taken together, these results indicate that beta, essentially, TNF-alpha, lesser extent, regulate expression plasma membrane cultured their depends part on synthesis. Therefore, macrophages, via products secretion acting 5'-nucleotidase, could modulate adenosine production capillaries.