Basis for a cheap method for detecting bacteria and assessing their antibiotic susceptibility in urine samples

摘要: Abstract Although molecular methods can now provide fast identification and the antibiotic susceptibility profile of infecting pathogens, these techniques are not affordable by a large majority clinical laboratories in developing countries be considered excessive for simple, community-acquired infections. Most antibiotics therefore prescribed empirically, which tends to avoid those drugs that face high resistance prevalence but could still used significant number patients. This is description basis fast, cheap method assessing presence bacterial infection its body fluids normally sterile, such as urine. The based on detecting decreased concentration glucose liquid media an indicator growth, effect upon this change growth arrest antibiotics. assess ca. 10 5  CFU/mL 4–6 h well phenotypes relevance. was tested 20 urine samples, comparing results with conventional culture assays; both proposed culture/antibiogram were agreement all cases. Materials needed would cost no more than US$5 per assay, it take 10 min set up, only requires small, fixed-temperature incubator. With minor modifications, much sensitive easy run and/or information specific bacteria.