Optimization of lipase activity from Rhizopus sp. in triglyceride hydrolysis using a modified simplex method
作者： Györgyi J. Kolossváry
DOI: 10.1016/S0032-9592(96)00005-2
关键词:
摘要: Abstract The simultaneous effect of reaction parameters and component concentrations on the microbial lipase from Rhizopus sp. was investigated. modified simplex optimization method applied to determine best operating conditions. activity measurement carried out with pH-stat where fatty acids liberated during enzymic hydrolysis olive oil substrate were titrated NaOH in an automatic tritrimeter. Two independent optimizations resulted a fully converged respect all parameters. Moreover, optimum parameter levels provided maximum activity, indicating that global has been found. 17·9 U/mg obtained at 40°C, pH 8·3, 41·8 mg/ml gum arabic, 0·6 sodium taurocholate. This response more than 100% better previous one by ‘one-factor-at-a-time’ method.
elsevier.com
sci-hub.se 参考文章(16)
Leitha D Bunch, Richard L Emerson, Serum lipase determination; four-hour technic with olive oil substrate. Clinical Chemistry. ,vol. 2, pp. 75- 82 ,(1956) , 10.1093/CLINCHEM/2.2.75
G.P. Fraser, A.D. Nicol, Studies on human pancreatic lipase. Clinica Chimica Acta. ,vol. 13, pp. 552- 562 ,(1966) , 10.1016/0009-8981(66)90158-6
E. Osterberg, A. -C. Blomstrom, K. Holmberg, Lipase Catalyzed Transesterification of Unsaturated Lipids in a Microemulsion Journal of the American Oil Chemists' Society. ,vol. 66, pp. 1330- 1333 ,(1989) , 10.1007/BF03022757
Gerald P. McNeill, Dieter Borowitz, Ralf G. Berger, Selective distribution of saturated fatty acids into the monoglyceride fraction during enzymatic glycerolysis Journal of the American Oil Chemists' Society. ,vol. 69, pp. 1098- 1103 ,(1992) , 10.1007/BF02541043
N.W. Tietz, Ermalinda A. Fiereck, A specific method for serum lipase determination Clinica Chimica Acta. ,vol. 13, pp. 352- 358 ,(1966) , 10.1016/0009-8981(66)90215-4
Stephen L. Morgan, Stanley N. Deming, Simplex optimization of analytical chemical methods Analytical Chemistry. ,vol. 46, pp. 1170- 1181 ,(1974) , 10.1021/AC60345A035
A. R. Macrae, Lipase-catalyzed interesterification of oils and fats Journal of the American Oil Chemists' Society. ,vol. 60, pp. 291- 294 ,(1983) , 10.1007/BF02543502
BAOKANG YANG, KIRK L. PARKIN, Monoacylglycerol Production from Butteroil by Glycerolysis with a Gel‐Entrapped Microbial Lipase in Microaqueous Media Journal of Food Science. ,vol. 59, pp. 47- 52 ,(1994) , 10.1111/J.1365-2621.1994.TB06894.X
L. H. Posorske, Industrial‐scale application of enzymes to the fats and oil industry Journal of the American Oil Chemists' Society. ,vol. 61, pp. 1758- 1760 ,(1984) , 10.1007/BF02582143
Gerald P. McNeill, Shoichi Shimizu, Tsuneo Yamane, Solid phase enzymatic glycerolysis of beef tallow resulting in a high yield of monoglyceride Journal of the American Oil Chemists' Society. ,vol. 67, pp. 779- 783 ,(1990) , 10.1007/BF02540491