Oligodendrocytes lack glycolipid anchored proteins which protect them against complement lysis. Restoration of resistance to lysis by incorporation of CD59.

摘要: Rat oligodendrocytes, which activate the classical pathway of complement in absence antibody, are highly sensitive a reactive lysis assay using human C5b6 and EDTA serum. Oligodendrocytes may be relatively deficient glycolipid-linked regulatory protein(s), since digestion with phosphatidylinositol-specific phospholipase C (PI-PLC) failed to increase their sensitivity serum, whereas complement-insensitive astrocytes, when treated PI-PLC, became strikingly sensitive. To test hypothesis that oligodendrocytes lack terminal molecule(s), erythrocyte CD59, recently described protein, was purified homogeneity. The biological activity preparation confirmed by reincorporating protein into guinea-pig erythrocytes through its glycolipid anchor, resulted dose-dependent protection against Incorporation 10(5) molecules CD59 rat good homologous (76%), significant cell (36%). Protection could reversed an antibody CD59.