Multimodal Imaging of Orthotopic Mouse Model of Endometrial Carcinoma

摘要: BACKGROUND: Orthotopic endometrial cancer models provide a unique tool for studies of tumour growth and metastatic spread. Novel preclinical imaging methods also have the potential to quantify functional characteristics in vivo, with relevance monitoring response therapy. METHODS: After orthotopic injection luc-expressing cells, eleven mice developed disease detected by weekly bioluminescence (BLI). In parallel same underwent positron emission tomography-computed tomography (PET-CT) magnetic resonance (MRI) employing 18F-fluorodeoxyglocose (18F-FDG) or 18F- fluorothymidine (18F-FLT) contrast reagent, respectively. The were sacrificed when moribund, post-mortem examination included macroscopic microscopic validation primary uterine tumours metastases. PET-CT was performed on patient derived model (PDX) generated from grade 3 endometrioid cancer. RESULTS: Increased BLI signal during accompanied increasing metabolic volume (MTV) MTV x mean standard uptake value (SUVmean) 18F-FDG 18F-FLT PET-CT, MRI conspicuously depicted tumour. At necropsy 82% (9/11) metastases applied methods. PET proved be good method detection tissue. CONCLUSIONS: We demonstrate that all modalities enable spread an mouse carcinoma. Both tracers, 18F-FLT, appear equally feasible detecting development represent, together MRI, promising tools patient-derived xenograft models.