Synthesis of beta-glucans in Prototheca zopfii. Evidence for the existence of a glycoprotein primer.
作者： Liliana A. RIVAS , Rafael PONT LEZICA
DOI: 10.1111/J.1432-1033.1987.TB10745.X
关键词:
摘要: Membrane preparations from the non-photosynthetic alga Prototheca zopfii incorporate glucose UDP-[3H]glucose into trichloroacetic-acid-insoluble fraction and polysaccharides insoluble in hot alkali. Time course pulse-chase experiments indicate that acid-insoluble was a precursor of alkali-insoluble fraction. Isolation 3H-labeled membrane or soluble showed only fractions were able to transfer radioactivity polysaccharides. Treatment glucosylated membranes with trypsin cellulase partially affect their ability, indicating internalized vesicles. Analysis vitro synthesized by enzymatic acid hydrolysis cellobiose present as radioactive sugars. Permethylation polysaccharide indicates 80% beta-1,4-bonded 20% beta-1,3-linkages. This found be identical cell-wall beta-glucan obtained vivo [Rivas, L.A. & Pont Lezica, R. (1978) Planta (Berl.) 165, 348-353].
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