Loading of Gemcitabine on chitosan magnetic nanoparticles increases the anti-cancer efficacy of the drug
作者： Maryam Parsian , Gozde Unsoy , Pelin Mutlu , Serap Yalcin , Aysen Tezcaner
DOI: 10.1016/J.EJPHAR.2016.05.016
关键词:
摘要: Targeted delivery of anti-cancer drugs increase the efficacy, while decreasing adverse effects. Among various systems, chitosan coated iron oxide nanoparticles (CsMNPs) gained attention with their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. This study aimed to cellular uptake efficacy Gemcitabine. CsMNPs were synthesized by in situ co-precipitation Gemcitabine was loaded onto nanoparticles. Nanoparticle characterization performed TEM, FTIR, XPS, zeta potential. release stability analyzed. The shown. Cytotoxicity free-Gemcitabine Gem-CsMNPs examined on SKBR MCF-7 breast cancer cells XTT assay. loading optimized as 30µM spectrophotometric analyses. Drug highest (65%) at pH 4.2, it 8% 7.2. is a desired characteristic since tumor-tissue endosomes are acidic, blood-stream healthy-tissues neutral. Peaks reflecting presence observed FTIR XPS. At neutral pH, potential increased after loading. TEM images displayed, 4nm uniform size-distribution have spherical shape. studied cell lines. IC50 value 1.4 fold 2.6 lower than free-Gem SKBR-3 lines respectively, indicating when Targetability field, stability, size distribution, characteristics this provides useful targeted system for therapy.
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