Ciprofloxacin-mediated induction of S-phase cell cycle arrest and apoptosis in COLO829 melanoma cells.

摘要: Abstract Background Low effectiveness of anti-melanoma therapies makes it necessary to search for new drugs that could improve or replace the standard chemotherapy. Fluoroquinolones are a group synthetic antibiotics, used in treatment wide range bacterial infections. Moreover, this class antibiotics has shown promising anti-tumor activity several cancer cell lines. The aim study was examine effect ciprofloxacin on viability, apoptosis and cycle distribution COLO829 melanoma cells. Methods Cell viability evaluated by WST-1 assay. cells exposed analyzed use fluorescence image cytometer NucleoCounter NC-3000. Results Ciprofloxacin decreased dose- time-dependent manner. For treated with 24 h, 48 h 72 h values IC50 were found be 0.74 mM, 0.17 mM 0.10 mM, respectively. oligonucleosomal DNA fragmentation observed when concentration 1.0 mM 72 h. At lower concentrations (0.01 mM 0.1 mM) arrested S-phase suggesting mechanism related topoisomerase II inhibition. demonstrated induced as result mitochondrial membrane breakdown. Conclusions obtained results compared data normal dark pigmented melanocytes potential anticancer drug vivo considered.