Sensitization of human malignant glioma cell lines to tumor necrosis factor-induced apoptosis by cisplatin.

摘要: Most tumors, including gliomas, are resistant to tumor necrosis factor (TNF) cytotoxicity unless protein or RNA synthesis is inhibited. We investigated the effects of combined use TNF-α and cisplatin (CDDP) on cultured malignant glioma cells, T98G, U373MG, A172, U87MG. All cell lines were sensitive treatment with CDDP but during 24 h-incubation. The had synergistic T98G U87MG not U373MG A172 cells. Sequential treatments showed that only pretreatment for 2 h followed by 22 was cytotoxicity. Annexin V-flow cytometry terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay can induce apoptosis in cells treated CDDP. Although express transcripts p75 TNF receptor 2, changes receptors found contribute susceptibility TNF-α. production interleukin-6, a representative cytoprotective cytokine, from stimulated suppressed actinomycin D, Our results indicate sensitize TNF-α-induced mechanism other than blocking production.