Loss of Antinociception Induced by Naloxone Benzoylhydrazone in Nociceptin Receptor-Knockout Mice

摘要: Nociceptin and nociceptin receptor, which show structural similarities to opioid peptides receptors, respectively, have been recently found constitute a novel neuromodulatory system. In the brain, however, physiological role of modulation via receptor is still unclear. Administered produces hyperalgesia hypolocomotion, whereas receptor-knockout mice no significant abnormalities in nociceptive thresholds locomotion. To clarify possible involvement regulation nociception locomotion, we made use knockout naloxone benzoylhydrazone (NalBzoH) identified originally as ligand for receptors. Experiments on cultured cells transfected with cDNA showed that NalBzoH competed [3H]nociceptin binding attenuated nociceptin-induced inhibition cAMP accumulation. Furthermore, behavioral studies demonstrated completely inhibited hypolocomotion. It therefore likely can act potent antagonist vivo. wild-type mice, induced antinociception but did not affect locomotor activity. contrast, changes locomotion were by NalBzoH. These results clearly suggest system takes part basal