Mucins : methods and protocols

摘要: 1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Karine Rousseau and Dallas M. Swallow 2. Gel-forming Cell-associated - preparation for Structural Functional Studies Julia R. Davies, Claes Wickstrom David J. Thornton 3. Detecting, Visualising Quantifying Ceri A. Harrop, Michael McGuckin 4. Mass Spectrometric Analysis of Core Proteins Mehmet Kesimer John K. Sheehan 5. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation Prediction Based on Random Peptide Substrates Thomas Gerken 6. Assembly Secreted Malin E.V. Johansson Gunnar C. Hansson 7. MUC1 Membrane Trafficking: Protocols Assessing Biosynthetic Delivery, Endocytosis, Recycling Release Through Exosomes Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey 8.Glycomic Work-flow O-linked Oligosaccharides Catherine Hayes, Szilard Nemes, Samah Issa, Chunseng Jin, Niclas G. Karlsson 9. O-Glycomics: Profoliling Mucin-type Glycans Isabelle Breloy 10. O-Glycoproteomics: Site-specific O-glycoprotein CID/ETD Electrospray Ionization Tandem Spectrometry Top-Down Glycoprotein Sequencing In-Source Decay MALDI Hanisch 11. Analysing Stephen D. Carrington, Jane Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, Anthony Corfield 12. Assessment Mucus Thickness Production In Situ Lena Holm Mia Phillipson 13. Preservation in Histrological Sections, Immunostaining Fixed Tissue Bacteria with FISH 14. vitro Measurements Secretion Colon Explants Jenny Gustafsson, Henrik Sjovall 15. Establishment Respiratory Air-Liquid Interface Cultures their Use Studying Production, Secretion, Function Hill Brian Button 16. from Human Bronchial Epithelial Cell Primary Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, Sheehan, William Davis 17. Intracellular Content Vivo Lucia Piccotti, Burton F. Dickey, Christopher Evans 18. Techniques Interactions Microbes Parasites Vitro Yong Sheng, Sumaira Z. Hasain, Chin Wen Png, Sara Linden 19. Expression Ocular Surface Epithelia Pablo Augueso Ilene Gipson