Regulatory effects of PRF and titanium surfaces on cellular adhesion, spread, and cytokine expressions of gingival keratinocytes.
作者： Mustafa Tunali , Gökhan KASNAK , Dareen Fteita , Olli Jaatinen , Eija Könönen
DOI: 10.1007/S00418-019-01774-8
关键词:
摘要: Dental implant material has an impact on adhesion and spreading of oral mucosal cells its surface. Platelet-rich fibrin (PRF), a second-generation platelet concentrate, can enhance cell proliferation adhesion. The aim was to examine the regulatory effects PRF titanium surfaces cellular adhesion, spread, cytokine expressions gingival keratinocytes. Human keratinocytes were cultured grade 4, 5 (Ti5), HA discs at 37 °C in CO2 incubator for 6 h 24 h, using either elutes titanium-PRF (T-PRF) or leukocyte platelet-rich (L-PRF), mammalian culture medium as growth media. Cell numbers determined Titer 96 assay. Interleukin (IL)-1β, IL-1Ra, IL-8, monocyte chemoattractant protein (MCP)-1, vascular endothelial factor (VEGF) expression levels measured Luminex® xMAP™ technique, spread by scanning electron microscopy. Epithelial most prominent Ti5 surfaces. L-PRF stimulated Both T-PRF activated IL-1 β, MCP-1, VEGF, being strongest activator. Titanium surface type role epithelial while determines response.
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