Intermediates and products formed during fatty acid α‐oxidation in cucumber (Cucumis sativus)

摘要: Fatty acid α-oxidation is an essential metabolic pathway both in plants and mammals which still not completely understood. We previously described purified enzyme cucumber has been used the present investigation of reaction mechanism. Free fatty acids, CoA thioesters, were found to undergo cucumber. 2-Hydroxy- 2-oxopalmitic acids identified as palmitic intermediates by high-performance liquid chromatography gas chromatography—mass spectrometry analysis subcellular 150,000×gmax pellets obtained differential centrifugation. Incubation with [1-14C]palmitic resulted formation above-described Cn−1 product, pentadecanal, 14CO2. Besides 14CO2, 14C-formate was product from fractions. Fe2+ stimulated 14CO2 production, addition ascorbate 2-oxoglutarate together optimal activities, suggesting a dioxygenase mechanism, shown mammals. NADPH and, lesser extent, NADH total plus production but had only slight or no effects on production. H2O2 showed concentration-dependent inhibitory effects, while FAD neither effect nor The results study demonstrate that capable oxidizing via 2-hydroxy- produce pentadecanal CO2. In contrast fraction, could formate convert into indicating two possible routes