Changes in nNOS and NADPH diaphorase in frog retina and tectum after axotomy and FGF-2 application.

摘要: We have shown previously that application of fibroblast growth factor-2 (FGF-2) to the cut optic nerve frog, Rana pipiens, augments survival retinal ganglion cells (RGCs). In this study, we examine effects axotomy and FGF-2 treatment upon distribution nitric oxide synthase (NOS) NADPH diaphorase (NADPH-d) activity in frog retina tectum. find NOS NADPH-d are largely absent from RGCs but present amacrine neurons retinorecipient tectal layers. Axotomy alone has little effect on expression or activity, apart slightly increasing levels a subpopulation arborize On sublamina inner plexiform layer. down-regulates up-regulates it tectum, particularly Electron microscopy neurofilament immunostaining tectum suggests increases number regenerating axons arriving at The probably indirect, being due retrograde signaling neurons, re-induction by arrival axons. At stage, appears unlikely these changes play role FGF-2's RGCs.