Genetic and genomic monitoring with minimally invasive sampling methods

摘要: The decreasing cost and increasing scope power of emerging genomic technologies are reshaping the field molecular ecology. However, many modern approaches (e.g., RAD‐seq) require large amounts high‐quality template DNA. This poses a problem for an active branch conservation biology: genetic monitoring using minimally invasive sampling (MIS) methods. Without handling or even observing animal, MIS methods collection hair, skin, faeces) can provide information on individuals populations. Such samples typically yield low‐quality and/or quantities DNA, restricting type that be used. Despite this limitation, is effective tool estimating population demographic parameters diversity in natural Genetic likely to become more important future as populations undergoing anthropogenically driven declines, which unlikely abate without intensive adaptive management efforts often include approaches. Here, we profile expanding suite platforms compatible with producing genotypes from MIS, considering factors such development costs error rates. We evaluate how powerful newquestions answered monitoring, abundance, structure relatedness. As period unusually rapid transition, also highlight importance legacy data sets recommend address challenges moving between traditional next‐generation platforms. Finally, consider could move beyond future. For example, assessing microbiomes epigenetic markers greater understanding relationship their environment. will enhance our ability investigate