作者: T. Staehelin , D.S. Hobbs , H. Kung , C.Y. Lai , S. Pestka
DOI: 10.1016/S0021-9258(19)68827-7
关键词:
摘要: Recombinant human leukocyte interferon produced in bacteria (IFLrA) was purified to homogeneity with the use of monoclonal antibodies against interferon. The exhibited a single band Mr = approximately 19,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid analysis and NH2-terminal sequence were consistent predicted from DNA. Some product contained methionine; terminal methionine removed rest chains.