Purification and characterization of recombinant human leukocyte interferon (IFLrA) with monoclonal antibodies.

作者: T. Staehelin , D.S. Hobbs , H. Kung , C.Y. Lai , S. Pestka

DOI: 10.1016/S0021-9258(19)68827-7

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摘要: Recombinant human leukocyte interferon produced in bacteria (IFLrA) was purified to homogeneity with the use of monoclonal antibodies against interferon. The exhibited a single band Mr = approximately 19,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid analysis and NH2-terminal sequence were consistent predicted from DNA. Some product contained methionine; terminal methionine removed rest chains.

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