Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis
作者： Eric Daugas , Santos A. Susin , Naoufal Zamzami , Karine F. Ferri , Theano Irinopoulou
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摘要: Apoptosis inducing factor (AIF) is a novel apoptotic effector protein that induces chromatin condensation and large-scale (∼50 kbp) DNA fragmentation when added to purified nuclei in vitro. Confocal electron microscopy reveal that, normal cells, AIF strictly confined mitochondria thus colocalizes with heat shock 60 (hsp60). On induction of apoptosis by staurosporin, c-Myc, etoposide, or ceramide, (but not hsp60) translocates the nucleus. This suggests only outer mitochondrial membrane (which retains intermembrane space) but inner hsp60 matrix) becomes permeable. The mitochondrio-nuclear redistribution prevented Bcl-2 specifically targeted membranes. pan-caspase inhibitor Z-VAD.fmk does prevent staurosporin-induced translocation AIF, although it inhibit oligonucleosomal arrests at an early stage. ATP deple...
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W. Zhu, A. Cowie, G. W. Wasfy, L. Z. Penn, B. Leber, D. W. Andrews, Bcl-2 mutants with restricted subcellular location reveal spatially distinct pathways for apoptosis in different cell types. The EMBO Journal. ,vol. 15, pp. 4130- 4141 ,(1996) , 10.1002/J.1460-2075.1996.TB00788.X
Hideki Sakahira, Masato Enari, Shigekazu Nagata, Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis Nature. ,vol. 391, pp. 96- 99 ,(1998) , 10.1038/34214
D.G. Brown, X.M. Sun, G.M. Cohen, Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. Journal of Biological Chemistry. ,vol. 268, pp. 3037- 3039 ,(1993) , 10.1016/S0021-9258(18)53654-1
Yoshihide Tsujimoto, Yutaka Eguchi, Shigeomi Shimizu, Intracellular ATP levels determine cell death fate by apoptosis or necrosis Cancer Research. ,vol. 57, pp. 1835- 1840 ,(1997)
Shigeomi Shimizu, Masashi Narita, Yoshihide Tsujimoto, Yoshihide Tsujimoto, Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC Nature. ,vol. 399, pp. 483- 487 ,(1999) , 10.1038/20959
Masato Enari, Hideki Sakahira, Hideki Yokoyama, Katsuya Okawa, Akihiro Iwamatsu, Shigekazu Nagata, A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD Nature. ,vol. 391, pp. 43- 50 ,(1998) , 10.1038/34112
A L Nieminen, A K Saylor, S A Tesfai, B Herman, J J Lemasters, Contribution of the mitochondrial permeability transition to lethal injury after exposure of hepatocytes to t-butylhydroperoxide Biochemical Journal. ,vol. 307, pp. 99- 106 ,(1995) , 10.1042/BJ3070099
Arimatti Jutila, Paavo K. J. Kinnunen, Marjatta Rytömaa, Detachment of Cytochrome c by Cationic Drugs from Membranes Containing Acidic Phospholipids: Comparison of Lidocaine, Propranolol, and Gentamycin Molecular Pharmacology. ,vol. 54, pp. 722- 732 ,(1998)
Yoshihide Tsujimoto, Yutaka Eguchi, Anu Srinivasan, Shigeomi Shimizu, Kevin J. Tomaselli, ATP-dependent steps in apoptotic signal transduction. Cancer Research. ,vol. 59, pp. 2174- 2181 ,(1999)
Willisa Liou, Hans J. Geuze, Jan W. Slot, Improving structural integrity of cryosections for immunogold labeling. Histochemistry and Cell Biology. ,vol. 106, pp. 41- 58 ,(1996) , 10.1007/BF02473201