Comparative Utility of Chloroplast DNA Restriction Site and DNA Sequence Data for Phylogenetic Studies in Plants

摘要: Restriction enzyme comparisons of plant genomes have been used widely to address systematic and evolutionary questions during the past 15 years. Most studies examined variation in chloroplast DNA (cpDNA) or nuclear ribosomal (rDNA) estimate phylogenetic relationships among species, genera, a few cases families plants (e.g., Jorgensen Cluster, 1988; Palmer et al., Olmstead Palmer, 1994). The restriction site approach also has examine intraspecific genetic diversity populations Neale 1986; Soltis 1989, 1991a, 1997; Whittemore Schaal, 1991; Fenster Ritland, 1992; Kim 1992a; Hong 1993; Petit Byrne Moran, 1994; Dong Wagner, McCauley, Mason-Gamer 1995; Bain Jansen, 1996). sequence data three characteristics that make them especially useful for analyses (Holsinger 1993). First, discrete character states can be scored unambiguously. Second, large number characters potentially obtained each taxon. Third, both types provide valuable information on extent nature divergence between sequences. None these features are present earlier methods, such as DNA—DNA hybridization.