Efficient Eradication of Subcutaneous but Not of Autochthonous Gastric Tumors by Adoptive T Cell Transfer in an SV40 T Antigen Mouse Model

摘要: In stomach cancer, there is a need for new therapeutic strategies, in particular the treatment of unresectable tumors and micrometastases. We investigated efficacy immunotherapy an autochthonous model gastric CEA424-SV40 T Ag (TAg) transgenic mice. Treatment against both s.c. induced by derived cell line mGC3 were assessed. wild-type mice, dendritic vaccine loaded with irradiated tumor cells combined CpG oligonucleotides efficient cytotoxic memory responses tumors. contrast, neither nor responded to vaccination TAg indicating tolerance SV40 TAg. To examine whether these mice principally accessible immunotherapy, splenocytes from immune adoptively transferred into Treated showed complete regression associated intratumoral infiltrates CD8 CD4 cells. same poorly infiltrated did not regress. Thus, even presence active anti-tumoral response, do respond immunotherapy. This first comparison adoptive transfer between transplanted animals. Our results suggest that cancer patients, strong anti-tumor response will efficiently penetrate absence additional strategies targeting microenvironment.