Prostaglandin E2 supports growth of chicken embryo intestinal organoids in Matrigel matrix

摘要: Investigating intestinal physiology in vitro remains challenging due to the lack of an effective primary enterocyte culture system. Recently developed protocols for growing organoids containing crypts and villus from adult mouse epithelium Matrigel present attractive alternative classical techniques. However, these approaches require use sophisticated expensive serum-free medium supplemented with epithelial growth factor (EGF), Wnt agonist (R-spondin 1), bone morphogenetic protein inhibitor (Noggin) high concentrations. Here we demonstrate that is possible isolated chicken embryonic create such organoid culture. Structures formed matrix first two days following isolation survive enlarge during ensuing weeks. They have appearance empty spheres comprise cells expressing cytokeratin (an cell marker), villin (a marker enterocytes), Sox-9 transcription characteristic progenitors stem crypts). With tissue as a source organoids, prostaglandin E2 R-spondin 1 Noggin promoting sustained survival spheroids.