Determination of zidovudine using anion exchange chromatography with integrated pulsed amperometric detection

摘要: Abstract A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography (HPAEC) has been developed with integrated pulsed amperometric (IPAD). Dionex AS18 (250 mm × 2 mm) AG18 (50 mm × 2 mm) columns 11 mmol/L NaOH solution were used separation. Multi-step potential waveform parameters optimized to maximize the signal-to-noise ratio (S/N). Utilizing an waveform, repeatability (intra-day) precision intermediate (inter-day) are obtained relative standard deviation (RSD) 1.88, 2.27, respectively. The limit quantification (LOQ) (LOD) found be 9.70, 3.0 ng/mL, respectively, correlation coefficients 0.9992 over concentration range 0.01–10 μg/mL. present was successfully applied determination human plasma. recoveries plasma sample spiked by 0.7 μg/mL, 2.7 μg/mL 95.3–101.5%, 2.54%, 2.21%,