L-Amino Acids Elicit Diverse Response Patterns in Taste Sensory Cells: A Role for Multiple Receptors

摘要: Umami, the fifth basic taste, is elicited by L-amino acid, glutamate. A unique characteristic of umami taste response potentiation 5’ ribonucleotide monophosphates, which are also capable eliciting an taste. Initial reports using human embryonic kidney (HEK) cells suggested that there one broadly tuned receptor heterodimer, T1r1+T1r3, detects L-glutamate and all other acids. However, growing evidence multiple receptors detect glutamate in oral cavity. While much understood about transduction, mechanisms for detecting tastes acids less well understood. We used calcium imaging isolated sensory cell clusters from circumvallate foliate papillae C57BL/6J T1r3 knockout mice to determine if might be involved detection Ratiometric with Fura-2 was study responses monopotassium L-glutamate, L-serine, L-arginine, L-glutamine, without inosine monophosphate (IMP). The results these experiments showed patterns varied significantly across cells. than synergistic a subset Along its role synergism, IMP alone large number Our data indicate non-synergistic mediated or possibly complex.