A vesicle superpool spans multiple presynaptic terminals in hippocampal neurons

摘要: Synapse-specific vesicle pools have been widely characterized at central terminals. Here, we demonstrate a pool that is not confined to synapse but spans multiple Using fluorescence imaging, correlative electron microscopy, and modeling of dynamics, show some recycling vesicles synapses form part larger “superpool.” The within this superpool are highly mobile rapidly exchanged between terminals (turnover: ∼4% total pool/min), significantly changing vesicular composition over time. In acute hippocampal slices the also feature native brain tissue. We available during stimulation, providing an extension classical pool. Experiments using focal BDNF application suggest involvement local TrkB-receptor-dependent mechanism for synapse-specific regulation presynaptic through control release capture or from extrasynaptic