Preliminary evaluation of the ligase chain reaction for specific detection of Neisseria gonorrhoeae.

摘要: Rapid identification of Neisseria gonorrhoeae in clinical specimens is essential for effective control. Traditional culture requires a minimum 24 h, and some harboring gonococci, the gonococci fail to grow or are misidentified. The recently described ligase chain reaction (LCR) highly specific sensitive DNA amplification technique which was evaluated as an alternative routine culture. Three LCR probe sets were used. Two directed against multi-copy Opa genes (Omp-II), while third set targeted multicopy Pilin genes. Each with 260 microorganisms including 136 global isolates N. gonorrhoeae, 41 meningitidis, 10 lactamica; 26 nonpathogenic strains; 47 non-Neisseria species that may reside specimens. Amplification products detected by using IMx format (Abbott Laboratories, Abbott Park, Ill.). Strains assayed at 270 cells per (approximately 6.7 x 10(4) CFU/ml) probes, producing signals least 21 15 times above background, respectively. In contrast, only background values observed when testing 124 nongonococcal strains 1.3 10(6) 3.2 10(8) CFU/ml). One hundred urogenital LCR, compared culture, three probes 100% (8 8) 97.8% (90 92), resulting agreement 98% (98 100). On basis results these preliminary studies, has potential be accurate rapid assay detection