Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.

摘要: Quantitative real-time PCR (qRT-PCR) is commonly used to measure gene expression further explore function, while suitable reference genes must be stably expressed under different experimental conditions obtain accurate and reproducible data for relative quantification. Taxol or paclitaxel an important anticancer compound mainly identified in Taxus spp. The molecular mechanism of the regulation taxol biosynthesis current research goal. However, case spp., few reports were published on screening as internal controls qRT-PCR. Here, eight selected candidate study. Common statistical algorithms geNorm, NormFinder, BestKeeper, ΔCt, RefFinder analyze from samples collected a cell line Taxus × media various tissues chinensis var. mairei. patterns TcMYC salicylic acid treatment differed significantly, with best worst line. This study screened out (GAPDH1 SAND) treatments reliable normalization qRT-PCR At same time, this will aid future biosynthesis-related can also directly other related species.