Egg extracellular coat proteins: from fish to mammals.

摘要: The extracellular coat surrounding fish (vitelline envelope; VE) and mammalian (zona pellucida; ZP) eggs is composed of long, interconnected filaments. Fish VE ZP proteins that make up the filaments are highly conserved groups related to each other, as well their amphibian avian egg counterparts. rainbow trout (O. mykiss) 3 proteins, called VEalpha (approximately 58 kDa), VEbeta 54 VEgamma 47 kDa). mouse (M. musculus) also ZP1 200 ZP2 120 ZP3 83 Overall, share approximately 25% sequence identity have features in common; these include an N-terminal signal sequence, a domain, consensus furin cleavage-site, C-terminal tail. VEalpha, VEbeta, trefoil or P-type domain upstream domain. very similar 65% identity) ZP2, whereas identity). Mouse synthesized secreted exclusively by growing oocytes ovary. Trout liver under hormonal control transported bloodstream assembled from VEalpha/gamma VEbeta/gamma heterodimers. ZP2/3 heterodimers crosslinked ZP1. Despite 400 million years separating appearance mice, change external internal fertilization development, many common structural features; do proteins. However, site synthesis has changed over time ovary, necessitating some changes region polypeptides regulates processing, secretion, assembly