Adhesion of ungerminated conidia of Colletotrichum graminicola to artificial hydrophobic surfaces

摘要: Conidia of Colletotrichum graminicola , the causal agent corn anthracnose, begin to adhere hydrophobic surfaces immediately after contact with surface and hours before onset germination. rarely adhered glass, a hydrophilic surface. The maximum percentage adhesion ungerminated conidia was approx. 30–40% on polystyrene Petri dishes 20–25% dimethyldichlorosilane (DMS)-coated glass slides. both attained within 30 min time contact. Thus, appear require for adhesion. extracellular mucilage that is produced in association shown not be involved Adhesion influenced by conidium concentration but age. Exposure different temperatures assays delayed ability adhere. respiration inhibitor, sodium azide transcription actinomycin D, had no effect In contrast, inhibitors glycoprotein transport (brefeldin A) protein synthesis (cycloheximide) reduced conidial 50%, respectively. Treatment pronase E prevented completely. If were pre-treated then washed free enzyme, 20 % population recovered treated Concanavalin A at 1 mg ml −1 also failed This partially reversed haptens glucose mannose. Together, these results suggest conidia.