Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio.

摘要: A possible way to improve the economic efficacy of acetone-butanol-ethanol fermentation is increase butanol ratio by eliminating production other by-products, such as acetone. The acetoacetate decarboxylase gene (adc) in hyperbutanol-producing industrial strain Clostridium acetobutylicum EA 2018 was disrupted using TargeTron technology. increased from 70% 80.05%, with acetone reduced approximately 0.21 g/L adc-disrupted mutant (2018adc). pH control a critical factor improvement cell growth and solvent 2018adc. regulation electron flow addition methyl viologen altered carbon flux acetic acid 2018adc, which resulted an 82% corresponding overall yield 57% 70.8%. This study presents general method blocking demonstrates potential