Inhibition by adenosine of reactive oxygen metabolite production by human polymorphonuclear leucocytes.

摘要: The stimulation of reactive oxygen metabolite production from human polymorphonuclear leucocytes by chemotactic peptide (fMet-Leu-Phe) was inhibited adenosine with a K0.5 0.6 microM. Dipyridamole (0.1 microM), an inhibitor uptake, did not prevent the effect adenosine. Non-metabolizable analogues could substitute for in potency order N-ethoxycarboxamideadenosine greater than 2-chloroadenosine L-N6-(phenylisopropyl)adenosine = D-N6-(phenylisopropyl)adenosine, which is characteristic A2 receptor. effects adenosine, and were reversed 8-phenyltheophylline. When endocytosis cytochalasin B, cells still susceptible to receptor agonists. 2-Chloroadenosine (10 microM) reduced activation respiration response 3.3-fold 1.4-fold. Activation latex beads or its analogues. It concluded that acts at antagonize those stimuli, such as peptide, cause increase intracellular free Ca2+ concentration.