摘要: Five different recombinant bioluminescent strains of Escherichia coli that contain the recA (responsive to DNA damage related stress), fabA (membrane damage), katG (oxidative grpE (protein and lac (constitutive expression, general toxicity) promoters fused bacterial lux operon from either Vibrio fischeri or Photorhabdus luminescens were used describe mechanisms toxicity several dibenzo-p-dioxins dibenzofurans have on bacteria, as well determine whether bacteria can sensitively detect presence these compounds. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) was found cause only DNA-related cells. However, four stress-responsive showed positive responses after addition l,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TCDD), whereas 2,3,7,8-tetrachlorodibenzo-p-furan (2,3,7,8-TCDF) caused DNA, oxidative, protein damage. 2,8-dichlorodibenzo-p-dioxin (2,8-DCDD) not induce any stresses tested for in this study, is, membrane, damage, indicating each congener might differentially interact with cell, stimulating differential stress within them. By using constitutive strain, we level cellular experienced due dioxins decreased order 2,3,7,8-TCDD (the most toxic), 1,2,3,4-TCDD, 2,8-DCDD, 2,3,7,8-TCDF. The 20% effective concentration (EC20), defined study chemical causes a decrease bioluminescence 60 min induction, 0.1 μg/L 2,3,7,8-TCDD, value is lower than other congeners demonstrates toxic compound study.
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