Phosphorylation of parathyroid hormone by human and bovine parathyroid glands.
作者： S A Rabbani , R Kremer , H P Bennett , D Goltzman
DOI: 10.1016/S0021-9258(17)43241-8
关键词:
摘要: Human and bovine parathyroid gland slices were incubated in vitro for varying time periods with inorganic 32P [35S]methionine or [3H]serine. Tissue was then extracted aqueous medium, hormone (PTH) purified. Incorporated found to coelute immunoreactive PTH multiple chromatographic systems, a peak of phosphorylated material could be resolved from the nonphosphorylated by reversed phase high pressure liquid chromatography. The amino acid composition both entities conformed that major glandular species PTH, accounted 10-20% total. A course revealed slow incorporation into hormone, after 4-h preincubation 32P, co-elution its precursor observed. Phosphoserine identified purified labeled Additionally dilute hydrolysis containing generated an 35S-labeled fragment which co-chromatographed. results are consistent phosphorylation on serine residues within NH2-terminal region human glands suggest prohormone occurs as well.
elsevier.com
sci-hub.st 参考文章(24)
M L Groves, E W Bingham, Properties of casein kinase from lactating bovine mammary gland. Journal of Biological Chemistry. ,vol. 254, pp. 4510- 4515 ,(1979) , 10.1016/S0021-9258(17)30040-6
W.R. Gray, [12] Dansyl chloride procedure Methods in Enzymology. ,vol. 11, pp. 139- 151 ,(1967) , 10.1016/S0076-6879(67)11014-8
B A Eipper, R E Mains, Phosphorylation of pro-adrenocorticotropin/endorphin-derived peptides. Journal of Biological Chemistry. ,vol. 257, pp. 4907- 4915 ,(1982) , 10.1016/S0021-9258(18)34612-X
R Kremer, H P Bennett, J Mitchell, D Goltzman, Characterization of the rabbit renal receptor for native parathyroid hormone employing a radioligand purified by reversed-phase liquid chromatography. Journal of Biological Chemistry. ,vol. 257, pp. 14048- 14054 ,(1982) , 10.1016/S0021-9258(19)45341-6
D. V. Cohn, R. R. Macgregor, L. L. H. Chu, J. R. Kimmel, J. W. Hamilton, Calcemic fraction-A: biosynthetic peptide precursor of parathyroid hormone. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 69, pp. 1521- 1525 ,(1972) , 10.1073/PNAS.69.6.1521
J. F. Habener, B. Kemper, J. T. Potts, A. Rich, Proparathyroid Hormone: Biosynthesis by Human Parathyroid Adenomas Science. ,vol. 178, pp. 630- 633 ,(1972) , 10.1126/SCIENCE.178.4061.630
Robert T. Sauer, Hugh D. Niall, Mary L. Hogan, Henry T. Keutmann, Jeffrey L. H. O'Riordan, John T. Potts, The amino acid sequence of porcine parathyroid hormone. Biochemistry. ,vol. 13, pp. 1994- 1999 ,(1974) , 10.1021/BI00706A033
Henry T. Keutmann, Mary M. Sauer, Geoff N. Hendy, Jeffrey L. H. O'Riordan, John T. Potts, Complete amino acid sequence of human parathyroid hormone. Biochemistry. ,vol. 17, pp. 5723- 5729 ,(1978) , 10.1021/BI00619A019
Henry T. Keutmann, Bess F. Dawson, Gerald D. Aurbach, John T. Potts, A biologically active amino-terminal fragment of bovine parathyroid hormone prepared by dilute acid hydrolysis. Biochemistry. ,vol. 11, pp. 1973- 1979 ,(1972) , 10.1021/BI00760A037
DAVID GOLTZMAN, EDWARD N. CALLAHAN, GEOFFREY W. TREGEAR, JOHN T. POTTS, Influence of guanyl nucleotides on parathyroid hormone-stimulated adenylyl cyclase activity in renal cortical membranes. Endocrinology. ,vol. 103, pp. 1352- 1360 ,(1978) , 10.1210/ENDO-103-4-1352