Neurotensin-loaded collagen dressings reduce inflammation and improve wound healing in diabetic mice

摘要: Impaired wound healing is an important clinical problem in diabetes mellitus and results failure to completely heal diabetic foot ulcers (DFUs), which may lead lower extremity amputations. In the present study, collagen based dressings were prepared be applied as support for delivery of neurotensin (NT), a neuropeptide that acts inflammatory modulator healing. The performance NT alone NT–loaded matrices treat wounds streptozotocin (STZ) induced mice was evaluated. Results showed not-cytotoxic up 72 h after contact with macrophages (Raw 264.7) human keratinocyte (HaCaT) cell lines. Moreover, those cells shown adhere without noticeable change their morphology. faster (17% area reduction) early phases wounded mice. addition, they also significantly reduced cytokine expression namely, TNF-α (p < 0.01) IL-1β decreased infiltrate at day 3 post-wounding (inflammatory phase). After complete healing, metalloproteinase 9 (MMP-9) skin (p < 0.05) increased fibroblast migration (collagen type I, alpha 2 (COL1A2) III, 1 (COL3A1)) deposition. These suggest collagen-based can effective release into enhancing process. Nevertheless, more prominent scar observed treated when compared treatment alone.