Storage Procedures Influence the Antioxidant Capacity of Bee Pollen

摘要: Bee products, including bee pollen, are known for their therapeutic and nutritional properties due to the presence of several nutrients (essential lipids amino acids, fat-soluble water-soluble vitamins bioelements) as well natural antioxidants, such flavonoids (e.g.: kaempferol, quercetin, isorhamnetin), phenolic acids (chlorogenic acid) catechins. Therefore, given its health beneficial properties, pollen is considered a valuable dietary supplement human diet, being currently marketed, used food supplement. Nonetheless, chemical antioxidant can be altered by factors, improper storage, type sample handling processing ageing. Thus, we have evaluated influence different conservation methods (freeze-drying, drying, freezing) in amount total phenolics capacity present 1-year samples, submitted storing procedures. Total content (TPC) was estimated spectrophotometrically using Folin-Ciocalteu method nuclear magnetic resonance spectroscopy (1H NMR); while (AC) determined ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) DPPH (2,2-Diphenyl-1-picrylhydrazyl) methods, that measure radical scavenging activities. Different solvent extraction procedures (ethanol acetone based) were also applied samples prior analysis. The results showed activity reduce considerably after one year especially when bee-pollen freezing