Studies on polynucleotides. LXVII. Initiation of protein synthesis in vitro as studied by using ribopolynucleotides with repeating nucleotide sequences as messengers.
作者： HP Ghosh , D Söll , HG Khorana , None
DOI: 10.1016/0022-2836(67)90142-8
关键词:
摘要: Abstract All of the 64 ribotrinucleotides were tested for binding fmet-tRNAF to ribosomes. The trinucleotides AUG, GUG, UUG, ACG, CUG, GCG, UGG, UCG and AGG stimulated binding, first three giving maximum effect. non-formylatable species (met-tRNAM) was recognized by triplets AUG GUG. Polypeptide synthesis in Escherichia coli cell-free system at 0.004 m -Mg2+ concentration proceeded only when messengers contained initiator codons; it also required presence additional stimulation obtained addition a protein fraction isolated from ribosomal washings. Poly r-UG containing repeating dinucleotide sequence directed fmet-(Cys-Val-)n, N-terminal established as fmet-Cys-Val-Poly r-AUG polymethionine met-tRNAM needed incorporation internal methionine. At low Mg2+ ion poly r-GUA specifically valine, dependent on fmet-tRNAF. Although triplet UUG ribosomes, r-UUG failed stimulate fmet no fmet-tRNAF-dependent polypeptide observed. From these results GUG GUA are concluded be codons initiation chains
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