Improving signal intensities for genes with low-expression on oligonucleotide microarrays.

摘要: DNA microarrays using long oligonucleotide probes are widely used to evaluate gene expression in biological samples. These oligonucleotides pre-synthesized and sequence-optimized represent specific genes with minimal cross-hybridization homologous genes. Probe length concentration critical factors for signal sensitivity, particularly when various levels being tested. We evaluated the effects of probe on intensity measurements a target sample. Selected single cell line were hybridized arrays four lengths concentrations determine how these parameters affected representing their expression. found that longer significantly increased signals low-expression target. High-expressing also boosted but lesser degree. Increasing concentration, however, did not linearly increase either low- or high-expressing conclude oligonuclotide better intensities low expressing arrays.