CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
作者： Toon Swings , David C. Marciano , Benu Atri , Rachel E. Bosserman , Chen Wang
DOI: 10.1038/S41467-018-04651-5
关键词:
摘要: CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting gene for modification, each genetic construct requires unique gRNA. By generating gRNA against the flippase recognition target (FRT) site, common element shared multiple collections, CRISPR-FRT circumvents this design constraint to provide broad platform fast, scarless, off-the-shelf engineering.
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