Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
作者： Satoshi. Takahashi , Katsuhiko. Murakami , Takashi. Anazawa , Hideki. Kambara
DOI: 10.1021/AC00079A016
关键词:
摘要: Gel capillary-array electrophoresis has been developed to achieve a high-sensitivity and high-throughput DNA analysis using real-time fluorescence detection. To eliminate excitation light scattering at the capillary surfaces irradiate all migration tracks simultaneously, tubes were removed from irradiated region. fragments eluted gel capillaries flowed into lower open capillaries. Multiple sheath flows of buffer solution around produced prevent band diffusion in gel-free The image was detected with two-dimensional detector coupled an image-splitting prism having four color filters
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