Effect of 1-alcohols on DNA condensation and protein folding

作者: S. Marchetti , S. Cinelli , G. Onori

DOI: 10.1016/J.CPLETT.2010.05.014

关键词:

摘要: The conformation response of calf thymus DNA and β-lactoglobulin (BLG) to solvent conditions altered by varying amounts ethanol (EtOH) tert-butanol (TBA) has been monitored viscosimetry circular dichroism measurements. A correlation between the effects EtOH TBA on condensation BLG denaturation clearly appears from data, suggesting that a similar molecular mechanism is responsible for alcohol these two very different systems processes. These alcohol-induced transitions appear in concentration range (alcohol mole fraction X = 0.22 0.07 TBA, respectively) where changes association state molecule occur (see compressibility IR data).

参考文章(38)
Calvin L. Stevens, Robert D. Elliott, Bradley L. Winch, Irwin L. Klundt, A New Rearrangement of α-Aminoketones Journal of the American Chemical Society. ,vol. 84, pp. 2272- 2274 ,(1962) , 10.1021/JA00870A062
G. Onori, M. D’Angelo, A. Santucci, Study of micelle formation in aqueous sodium n -octanoate solutions Steinkopff. pp. 154- 157 ,(1994) , 10.1007/BFB0115155
D. Eagland, Nucleic Acids, Peptides, and Proteins Springer, Boston, MA. pp. 305- 518 ,(1975) , 10.1007/978-1-4684-2958-9_5
A. Bonincontro, A. De Francesco, M. Matzeu, G. Onori, A. Santucci, Conformational changes of lysozyme in water-ethanol mixtures Colloids and Surfaces B: Biointerfaces. ,vol. 10, pp. 105- 111 ,(1997) , 10.1016/S0927-7765(97)00055-6
Patricia G. Arscott, Chenglie Ma, Jay R. Wenner, Victor A. Bloomfield, DNA condensation by cobalt hexaammine (III) in alcohol-water mixtures: dielectric constant and other solvent effects. Biopolymers. ,vol. 36, pp. 345- 364 ,(1995) , 10.1002/BIP.360360309
G. Onori, A. Santucci, Effect of temperature and solvent on the critical micelle concentration of sodium dodecylsulfate Chemical Physics Letters. ,vol. 189, pp. 598- 602 ,(1992) , 10.1016/0009-2614(92)85257-B
S. Cinelli, G. Onori, A. Santucci, Effect of Aqueous Alcohol Solutions on the Thermal Transition of Lysozyme: A Calorimetric Study Journal of Physical Chemistry B. ,vol. 101, pp. 8029- 8034 ,(1997) , 10.1021/JP971399P
Francesca Bellezza, Antonio Cipiciani, Stefania Cinelli, Giuseppe Onori, Influence of alcohols and osmolytes on thermal stability and catalytic activity of myoglobin: Co-solvent clustering effects Chemical Physics Letters. ,vol. 482, pp. 139- 142 ,(2009) , 10.1016/J.CPLETT.2009.09.103