Long-term outcome and alloantibody production in a non-myeloablative regimen for induction of renal allograft tolerance

摘要: Background Multilineage chimerism and long-term acceptance of renal allografts has been produced in non-human primates conditioned with a nonmyeloablative regimen. Our study was undertaken to evaluate the immunological pathological status survivors define role splenectomy primarily vascularized kidney Method Monkeys were treated basic regimen, including: total body irradiation, thymic antithymocyte globulin, donor bone marrow transplantation, 4-week course cyclosporine after which no further immunosuppression given. They divided into four groups according timing transplantation (KTx) as follows; group A (n=13): KTx on day (day 0); B (n=3): 0 without splenectomy; C (n=7): but delayed until 3 16 weeks post-donor transplantation; D both 120 transplantation. Results In A, 11 13 monkeys developed 9 achieved survival 4 70 months evidence chronic vascular rejection. Alloantibodies detected only one survivor. contrast, all three alloantibodies rejected their allografts. C, alloantibody production observed two that had chimerism. D, recipients sensitized rapidly Conclusions 1) Production anti-donor antibody prevented most mixed regimens at time 2) If is not included initial conditioning induction cell tolerance less likely result late onset allograft 3) Immediate recipient essential for specific hyporesponsiveness by