Exciting Prospects for Precise Engineering of Caenorhabditis elegans Genomes with CRISPR/Cas9

摘要: With remarkable speed, the CRISPR-Cas9 nuclease has become genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because Cas9 can be guided by a single, short RNA (sgRNA) to introduce double-strand breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed eight different research groups, six which are published in GENETICS, modify Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering levels playing field experimental geneticists.